1. Please state project objectives and what work was done this quarter to address them: Project # 21-025 continues its focus on developing HLB-resistant and other improved citrus cultivars to combat the devastating effects of HLB on the Florida citrus industry. The project employs transgenic and non-transgenic approaches and conducts experiments at the secure field site of the USDA/ARS Picos Research Farm. The goal remains to provide sustainable solutions to combat HLB, safeguard the citrus industry, and support economic citrus production.This quarter, the primary emphasis was on analyzing the data collected from the ongoing field trials for various HLB-resistant candidates. Researchers thoroughly evaluated the efficacy of different strategies to combat HLB and its psyllid vectors based on the data obtained. The test site continued to receive the necessary horticultural care and maintained exposure to HLB and psyllid vectors to ensure realistic evaluations of the candidates’ resistance. The team continued to adhere to BRS permits and underwent inspections to ensure compliance with regulatory requirements. 2. Please state what work is anticipated for next quarter: Next quarter, the team will finalize the analysis of data from the current field trials. Based on the results, the project will make recommendations for advancing promising HLB-resistant candidates to larger replicated trials for further validation. Researchers will have continued access to the test site for any additional data collection and analysis requirements. There will be an update to trangenic field markers sinage and labeling to insure ease of finding material and compliance with BRS permits. We will also be conducting a transgenic field day with all of the cooperators that have material at the site. The project team will also initiate the process of requesting a six-month no-cost extension to the project timeline. 3. Please state budget status (underspend or overspend, and why): This quarter, there was an underspend mainly due to underspend in the previous quarters. Given the temporary disruptions caused by changes in PI and leadership at USHRL, the project team is requesting a six-month no-cost extension to the project timeline. The extension will allow sufficient time to complete the ongoing field trials, finalize data analysis, and make informed recommendations for advancing potential HLB-resistant candidates. The no-cost extension will ensure the project’s successful completion without additional financial burden to the funding agency. 4. Please show all potential commercialization products resulting from this research, and the status of each: The research has shown promising results in identifying HLB-resistant transgenic and non-transgenic citrus cultivars. Some of the candidate materials display a high likelihood of being suitable for immediate commercial citrus production. These HLB-tolarant cultivars could become valuable assets to the citrus industry, providing a sustainable solution to combat the devastating impact of HLB. Other materials may serve as important breeding parents for further improvement in HLB resistance and other commercially significant traits.The data gathered from the field trials will drive recommendations for advancing the most promising materials to larger replicated trials, ensuring the development of commercially viable HLB-resistant citrus varieties.