Testing continues with L. crescens assay through CRDF research contract. As of the end of March, there have been 804 compounds tested since the start of the project. The new additional compounds tested included tetracycline derivatives from two companies, Echelon and Paratek, antimicrobial peptides, bacterial fermentation products, GRAS oils, and a zinc product from a collaborative lab at UCF. Work was started again with samples that were collected from a collaboration experiment with Dr. Mirkov at Texas A&M. They set up a trial with tomato plants and the relative pathogen of citrus greening, Liberibacter solancearum. This trial was designed to test the effectiveness of oxytetracycline as a treatment sprayed to the infected plants as a foliar treatment. Other antimicrobials were tested at the same time, including Mycosheild, streptomycin, and emulsion of thyme oil. The intention is to determine the bacterial load at various time points of the experiment using qpcr technology. Work is underway to optimize the procedure from published work (Li et al 2009), as the initial trials following the paper�s protocol with standard curves did not work. Work also continues with Dr. Sharma�s group at UF PERC to use GC-MS to detect carvacrol after spraying to citrus plants. We have performed experiments on Duncan and Valencia citrus varieties with carvacrol, and multiple surfactants. So far no oil is being detected after foliar spray in the trunk of the plants. Trials have entailed spraying once a day in the morning for 3 days, and collecting and processing material on the 4th day. Care is taken to protect the trunk and soil and sprayed carefully tilted to prevent contamination with the oil to those areas. One trial done on Valencia tested the bark painting method, and samples were taken above and below treatment area, as well as in the leaves. When painting the trunk we used a q-tip dipped in the formulation, and thoroughly covered the treatment area 3x a day for 3 days. Plans for more trials are underway.