Since the last report, 84 more compounds have been tested in L. crescens assay to search for a treatment for citrus greening, bringing the total tested to 888. Most of the testing was continuing with bacterial fermentation products from major companies. Multiple compounds have been tested from smaller start-up corporations that have been having mixed success. Optimization of qpcr methods has encountered many problems, such as contamination, standard curve dilutions, efficiency of reactions, and reproducibility of the results. It turns out the qpcr machine was producing a systematic error and needed to be repaired. After repair, optimization continued, however research also underwent for alternative methods. A few papers were found that utilized the SYBR green chemistry vs the TaqMan chemistry for Lso detection. We decided to test out the alternative methods in hopes of consistent success. Unfortunately those methods proved to be just as challenging. With one good standard curve, it was decided to return to the TaqMan chemistry and a few more optimization runs were performed. Results have been reliable and work on the samples is underway. Trials with PERC group also continue, this time with much higher concentrations of carvacrol. Results are interesting, but some results seem to indicate contamination with the negative control, however results are still being analyzed. There is one more trial being planned with this project.