A number of successes have been documented at the Picos Test Site funded through the CRDF.� The UF Grosser transgenic effort has identified promising material, eliminated failures, and continues to replant with new advanced material (Grosser, personal comm.).� Using trees planted at the test site, transgenic overexpression of an Arabidopsis defense gene was reported to enhance citrus HLB resistance (Dutt et al., 2015).� The ARS Stover transgenic program has trees from many constructs at the test site and is seeing some modest differences so far, but new material planted this spring that has shown great promise in the greenhouse (Hao, Stover and Gupta, 2016).� �A trial of more than 85 seedling populations from accessions of Citrus and citrus relatives (provided as seeds from the US National Clonal Germplasm Repository in Riverside, CA) has been underway for 6 years in the Picos Test Site. P. trifoliata, Microcitrus, and Eremocitrus are among the few genotypes in the citrus gene pool that continue to show substantial resistance to HLB (Ramadugu et al., 2016), P. trifoliata displayed reduced colonization by ACP (Westbrook et al., 2011), and measures of HLB-tolerance were associated with percentage citron in accession pedigrees (Miles et al., 2017). A UF-Gmitter led association mapping study is underway using the same planting, to identify loci/genes associated with HLB- and ACP-resistance.� A broad cross-section of other Poncirus derived material is being tested by USDA-ARS-Riverside and UCRiverside.�More than 100 citranges, from a well-characterized mapping population, and other trifoliate hybrids (+ sweet orange standards) were planted in a replicated trial in collaboration with Fred Gmitter of UF and Mikeal Roose of UCRiverside.� Plants were monitored for CLas titer development and HLB symptoms. Data from this trial should provide information on markers and perhaps genes associated with HLB resistance, for use in transgenic and conventional breeding. A manuscript reporting identified HLB resistance associated QTLs has been published (Huang et al., 2018). David Hall assessed ACP colonization on a subset of plants and further documented host morphological traits associated with ACP-colonization in Poncirus (Hall et al., 2017a&b). Several USDA citrus hybrids/genotypes with Poncirus in the pedigree have fruits that approach commercial quality, were planted within the citrange site.� As of April 2014 at the Picos Test Site, several of these USDA hybrids had grown to a height of seven ft (one now released as US SunDragon), with dense canopies and good fruit set, while sweet oranges were stunted (3 ft) with very low vigor.� These differences largely continue and the observations have encouraged aggressive use of this and other trifoliate hybrids as parents (Stover et al., unpublished).� �A Fairchild x Fortune mapping population was planted at the Picos Test Site in an effort led by Mike Roose to identify loci/genes associated with tolerance.� This replicated planting also includes a number of related hybrids (including our easy peeling remarkably HLB-tolerant 5-51-2) and released cultivars.� HLB phenotyping and growth data have been collected and genotyping will be conducted under a new NIFA grant.�Valencia on UF Grosser tertazyg rootstocks have been at the Picos Test Site for several years, having been CLas-inoculated before planting, and several continue to show excellent growth compared to standard controls (Grosser, personal comm.).�Numerous promising transgenics identified by the Stover lab in the last two years have been propagated and will be planted in the test site.� New transgenics from Jeffrey Jones and Zhonglin Mou of UF, Tim McNellis of PSU will be planted in the next month.� Availability of this resource will continue to b