The Southern Gardens Diagnostic Laboratory (SGDL) has been in operation since October 2006. To date, the SGDL has received and assayed 168,469 grower samples, and approximately 35,600 additional samples from various sources (research samples, psyllid samples, and samples from Southern Gardens Citrus) for a total of over 204,000 samples since the SGDL began receiving samples. For the period of time covered by this report (July 1, 2010 to March 31, 2011), a total of 25,247 grower samples, 3,337 psyllid samples and 9,588 research and Southern Gardens samples were received and analyzed for a total of 38,172 samples for the period. Based on the number of samples received to date, it is expected that the sample load will approach the budgeted sample load of 45,000 allocated for the period of the funding. Although the total sample volume may not be changing drastically, one thing that is changing, however, is the mix of sample type/sample submitter. Although the number of grower samples is declining slightly, the number of research samples is increasing. The research samples are being submitted by private, state, and federal researchers in support of their research work. The research samples tend to be from large-scale field trials, survey projects, and from chemical/nutritional management projects. We expect this trend to continue. Among the reasons for this are continuity in testing and the fact that the cost of testing is being supported by the industry. Progress has been made in automating a portion of the DNA extraction process. Programming of the liquid handling robot has largely been completed and several hundred samples have been run using the automated extraction process and compared to the standard extraction protocol. Although generally the results from both methods are similar, we have had some problems with consistency using the automated method. Improving the consistency will be the major focus going forward. Plans are being made for the national psyllid ring test. It is hoped that the samples will be sent out during the final quarter of the funding period. It is expected that a large portion of the US labs currently doing HLB testing will participate as well as labs in Mexico and possibly elsewhere.