This project is a continuation of the funding that has been provided to Southern Gardens Citrus (SGC) to provide growers, researchers and private companies a laboratory to detect the pathogen that causes huanglongbing (syn. citrus greening). For the first quarter of the second year of funding (July 1, 2016 – September 30, 2016), 8,407 samples were processed. This compares to 6,951 for the first quarter of 2015. Of the samples processed for the first quarter of year 2 funding, 7,749 of the samples were plant samples and 658 samples were psyllid samples. Cumulative for the funding provided to date (5 quarters) a total of 41,356 samples have been run. This represents a 65% increase in sample load compared to what was budgeted for the period. During year 2 of the funding, we plan on adapting a PMA protocol that will allow the differentiation of live versus dead cells in the qPCR determination. This protocol will be implemented on a limited basis for specific trials. Once the procedure is validated, it may be made available on a limited basis to a wider group of researchers.