Support role of the Citrus Core Transformation Facility remains crucial for research leading to production of Citrus plants that may be tolerant or resistant to diseases

Support role of the Citrus Core Transformation Facility remains crucial for research leading to production of Citrus plants that may be tolerant or resistant to diseases

Report Date: 10/12/2015
Project: 15-033C   Year: 2015
Category: Horticultural & Management
Author: Vladimir Orbovic
Sponsor: Citrus Research and Development Foundation

For the last three months, Core Citrus Transformation Facility (CCTF) continued to provide its service for production of transgenic Citrus plants. Within this period of time, there were only two new orders placed but three clients requested work on three previously placed orders bringing total number to five. Probably as a result of recent Knowledge Mapping meeting organized by CRDF there is an increased interest for transgenic plants and CCTF was already informed of eight new incoming orders. That does not include additional orders from another client with whom CCTF manager communicated for the last three months. Forty plants were produced during the last quarter which represents lower productivity than usual. Partially, this is the result of work on group of three orders that have not yielded any plants. Another reason is the low quality of seeds used to obtain seedlings as starting material for experiments. High majority of produced plants belong to eight orders placed within the last 12-15 months. Five plants belong to three older orders. Most of the transgenic plants are Duncan grapefruit and Valencia orange and one of them is Carrizo citrange. The work has begun on determination of level of expression of AtNPR1 gene in rootstock plants produced for CRDF. Once all plants produced by CCTF and Mature tissue lab are processed, those with the highest expression levels will be selected for further use and propagated.


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