T-SOL (TM) is a new class of Zn chelate based antimicrobial compounds developed against citrus pathogens particularly HLB bacteria. T-SOL is designed to be systemic with the potential to be effective against citrus canker and HLB. Tasks completed in this reporting period: 1) Six different formulations of T-SOL (varying the chelating components) were prepared (metallic zinc concentration 20,000 PPM in 16 liters) and sent to field trials on the months of April and August of 2015. 2) Preliminary greenhouse studies performed in X. citri subsp., citri infected citrus trees suggested that 2 out of 3 T-SOL formulations (3 variants of TSOL formulations used in this study) significantly reduced the canker leaf lesions to ~60% in comparison to untreated control (100%), demonstrating at least locally systemic activity. 3) In vitro plant uptake studies were conducted with four different concentrations of T-SOL UP (800, 500, 300 and 100ppm). T-SOL UP is used in this set of studies but the same studies will be replicated in the next reporting periods with all the other 5 formulations mentioned above. Tomato plants (Solanum lycopersicum, used as model system) treated through foliar spray application method with the above-mentioned concentration of T-SOL for 24hours. After 24hours of treatment plants were sampled for digestion and analysis of zinc uptake by different parts of plants (whole plant, leaf, stem, root, phloem and xylem) through atomic absorption spectroscopy (AAS). Our result suggested maximum uptake of zinc by whole plant, leaf, stem, root and xylem at 500ppm treatment. Systemic uptake of zinc by phloem was highest at 300ppm while lowest uptake was observed at 500ppm (leaf phytotoxicity was observed at 500 ppm). Overall, treatment with T-SOL at all the different concentrations (100, 300, 500 and 800 ppm) showed definitive uptake of zinc chelate ranging from 0.3 to 7.1 mg per gram dry weight by different parts of the plant when compared to untreated plants (through the stomatal pores regardless of treatment concentration of metallic zinc). 4) Fourier Transform Infra red spectroscopy (FTIR) studies confirmed the formation of zinc chelate. Most of the resemblances were found in the fingerprint zones of the spectra. In the coming reporting periods, optimization of the synthesis protocol of the other five variants of T-SOL will be performed and their antimicrobial and phytotoxicity studies will be conducted to narrow down the most effective T-SOL variant that can be later taken to green house and field trial studies.