1. Please state project objectives and what work was done this quarter to address them: This report covers the period of June 1, 2021 – August 31, 2021. The objective of this project is to test transgenic ‘Ducan’ grapefruit trees expressing an anti-HLB antibody fused to the FT (Flowering Locus T) protein (FT-scFv protein). We have inoculation tests completed using graft transmission and Asian citrus psyllid (ACP) transmission of hte pathogen (Candidatus Liberibacter asiaticus, or CLas), and a natural infection trial in a grove at USHRL. The major accomplishment in this quarter was that we received data returned form Southern Gardens on all the infected trees from our greenhouse tests and those from a field trial natural inoculation. These are PCR data showing CLas titers in the infected plants. 2. Please state what work is anticipated for next quarter:We will use the next quarter to analyze the data on CLas titers in all our test trees and compare transgenics with controls. 3. Please state budget status (underspend or overspend, and why):The project is running under budget due to support received from Penn State for graduate student Chad Vosburg. We plan to request an additional no-cost extension to cover publication costs only into 2022.