1. Please state project objectives and what work was done this quarter to address them: This report covers the period of December 1, 2021 – February 28, 2022. The objective of this project is to test transgenic ‘Ducan’ grapefruit trees expressing an anti-HLB antibody fused to the FT (Flowering Locus T) protein (FT-scFv protein). Chad Vosburg graduated in December, 2021 with his M.S. degree in Plant Pathology and his M.S. Thesis was approved. The PI reviewed the M.S. thesis elements related the HLB resistance tests that we completed in the greenhouse and an ongoing test in the field at Fort Pierce. An initial draft of a manuscript describing these results was prepared by the PI. 2. Please state what work is anticipated for next quarter:In the next quarter, the PI will finalized the manuscript in collaboration with Chad Vosburg and other co-workers. The manuscript will be submitted by the PI to a peer-reviewed journal for review in the next quarter. We anticipate requesting another no-cost extension to allow us to use grant funds to pay publication costs for the manuscript. Review, acceptance and publication could take several additional months, at least. 3. Please state budget status (underspend or overspend, and why):The project remains underspent in part due to support for graduate stipends and tuition waviers from the Plant Pathology & Environmental Microbiology Department of Penn State and the Penn State Graudate School. We have sufficient funds remaining for publication costs for the upcoming manuscript.