The present report covers the period of December 1, 2019 through February 29, 2020. During this period, the M.S. student on the project (Mr. Chad Vosburg) made a visit to the USDA United States Horti-cultural Laboratory in Fort Pierce, FL, in December and January. In collaboration with USDA personnel, in particular, Dr. Greg McCollum and Dr. Tim Gottwald and members of their laboratories, Chad prepared for and helped set up psyllid-mediated HLB infections for 20 plants for each of two transgenic anti NodT-FT-scFv lines of ‘Duncan’ grapefruit. Asian Citrus Psyllid (ACP) mediated transfer was done in tents in the greenhouse. The transfer was deemed complete after psyllid feeding and reproduction on the plants was observed. The trees are now in the greenhouse. Time zero DNA samples were taken from the trees, and the samples were analyzed by qPCR for ‘Candidatus Liberibacter asiaticus’ (CLas) DNA. No CLas DNA was detected in the test plants. Control untransformed ‘Duncan’ grapefruit trees were also included in the experiment. All trees used for this experiment are on their own roots (ungrafted). In addition, transgenic FT-scFv buds were grafted onto rough lemon roostocks heavily infected with CLas during this trip by Chad. Populations of two high-expressing FT-scFv lines have been started in a field trial at the Pecos Farm site of the United States Horticultural Research Laboratory at Fort Pierce, FL, and these trees have begun to grow as of the end of February, 22020. This field trial includes non-transgenic ‘Duncan’ trees as well. Tree performance measurements according to standard parameters have been initiated for all trees. The trees for the outdoor trial were also tested for CLas DNA by qPCDR and found to be negative at the time of planting.