Under Objective 1 (Survey and confirmation of HLB in seedlings from HLB-affected trees) mild to severely affected fruit from the an HLB-affected Pineapple orange and Murcott tangor fruit were collected from groves in Collier County, FL and freshly extracted seed planted in the greenhouse for HLB detection in emergent seedlings. Under Objective 2, (Thermotherapy of HLB-affected seed) subsets of these same seed sources were treated at 125, 130 and 135F to test the effect of heat treatment on detection of HLB in seedlings. Pineapple orange and Murcott seedlings will be tested by PCR and any PCR products detected will be cloned and sequenced. Under Objective 3 (Follow-up on HLB-positive trees from DPI annual survey), DPI survey of seed source trees in Florida nurseries has, thus far, not detected seed trees PCR positive for HLB.