1. Please state project objectives and what work was done this quarter to address them: This is a new 3-year project. The goal is to evaluate new anti-CLas compounds in Florida singly and in combination with OTC to determine their effects on HLB tree health, yield, and injury. The study objective are:Objective 1: Pre-screening and quality control (purity, solubility, and bioactivity) of selected anti-CLas leads. (Initiated). Objective 2: Multi-year field evaluation of new chemicals, OTC, and combinations by trunk injections. (Initiated). We identified five new active ingredients for which synthesis and sourcing in sufficient amounts needed for the field trials were completed. Quality control experiments were initiated to ensure batch purity, solubility, and bioactivity, as determined using the CLas-citrus hairy root bioassays per our established methods (Irigoyen et al., 2020; Mandadi et al., 2020). Site and tree selection for the field trials in Florida have been initiated. The field trial will likely use 6-8-year-old HLB-affected sweet orange (Valencia) trees, either at the UF/SWFREC or a commercially operated citrus grove that has not already treated them with OTC in the prior year. 2. Please state what work is anticipated for next quarter: In the next quarter, we will complete all quality controls and initiate trunk injections in Florida. The plan is to inject the trees in the Spring (April or May 2024) after the new flush has hardened to ensure optimal delivery of the injected material. General grove management will follow practices determined by the grove operators. Baseline horticultural measurements (tree height, canopy spread, trunk diameter) will be conducted before injections, and then annually. 3. Please state budget status (underspend or overspend, and why): Ontrack with spending. 4. Please show all potential commercialization products resulting from this research, and the status of each: By the end of the study, we anticipate identifying one or more new active ingredients that improve the HLB-affected tree health and fruit yield. The new chemicals could be developed as commercial products singly or combined with OTC for HLB management.