Objective 1. (Greenhouse experiment): PCR samples were taken from every tree and sent to the Southern Gardens diagnostic laboratory to determine HLB status. Nutritional profile as well as infection status of all grafted plants were analyzed in this quarter. qPCR values indicated that there was no significant differences in the infection rate amongst the different treatments at this time. The Harrell�s nursery mix supplemented with Boron has lower overall infection rates as estimated by higher Ct values in all the rootstocks except UFR15, where the nursery mix supplemented with Manganese performed better. It is still too early to see much treatment and rootstock differences. Objective 3: To evaluate the effect of complete, balanced and constant nutrition on HLB-affected mature trees (composition, delivery and economics). The total 2nd year fertilizer applications were completed. Year 2 yield and fruit quality data collection will begin this quarter. Ojective 5. (funded by Lee Groves, using donated fertilizer products): Alligator Vernia/Rough Lemon Enhanced Nutrition Experiment � Treatments: 6 tree plots (randomized), 2 plots per treatment � treatments 2 times per year. Potential breakthrough – qPCR was run on each tree – significant bacterial titer differences were found among the treatments. The best treatment was a 4x overdose of manganese, producing an average ct value of 32.75; whereas the control tree average ct value was 23.19 (indicating severe infections). Seven of twelve trees in the high manganese treatment exhibited ct values of 32 or above, and were considered not to have an active infection; whereas no control trees reached 32. Other treatments containing high manganese also did much better than the controls, except for the treatment with both 4x manganese and 4x boron. This level of boron seemed to be antagonistic to the uptake of manganese, as demonstrated by the leaf mn content data. Yield and fruit quality data from these plots are being collected this quarter – results should be quite interesting.