Understanding the role of systemic acquired resistance (SAR) in enhancing tolerance to HLB in the Parson Brown sweet orange

Understanding the role of systemic acquired resistance (SAR) in enhancing tolerance to HLB in the Parson Brown sweet orange

Report Date: 10/07/2021
Project: 20-014   Year: 2021
Category: Horticultural & Management
Author: Manjul Dutt
Sponsor: Citrus Research and Development Foundation

1. Please state project objectives and what work was done this quarter to address them:Project objectives:Objective 1: Determine the effect of systemic acquired resistance (SAR) in enhanced tolerance to HLB  Objective 2: Monitor tree health and HLB levels Objective 3: Conduct Juice analysis and evaluate quality parameters from selected Parson Brown trees.  In this quarter we recorded a downregulation in the expression of CsPR1 and CsPR2 when compared with the first sampling in May in most of the experimental sites Parson Brown trees from two locations in Polk county had enhanced expression of CsPR1. There was no significant difference between Hamlin and Parson Brown performance from trees in the Immokalee site during July sampling. In addition to gene expression, samples have been collected for the following physiological parameter evaluation: Total chlorophyll content, starch content, alpha Amylase content, beta Amylase content, Carbohydrate content, Antioxidant content, Total phenolic compound content, Ascorbate content. A magazine article has been published in the Citrus Industry magazine to enhance public awareness on the parson brown oranges.  2. Please state what work is anticipated for next quarter:In the next quarter, we will again sample the same groves and collect similar leaf samples and evalute them. HLB levels will be evaluated using qPCR. Juice quality from all sites will be evaluated through the CREC pilot plant.  3. Please state budget status (underspend or overspend, and why):We are on target with the spending for this project.     


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