1. Please state project objectives and what work was done this quarter to address them:Project objectives:Objective 1: Determine the effect of systemic acquired resistance (SAR) in enhanced tolerance to HLB Objective 2: Monitor tree health and HLB levels Objective 3: Conduct Juice analysis and evaluate quality parameters from selected Parson Brown trees. We have identified and collected leaf samples in the spring from 8 Parson Brown groves. Groves are spread all around the major citrus growing regions. We sampled from 3 groves in polk county, 2 groves in Highlands county, 1 grove in St. Lucie county, 1 grove in glades county and 1 grove in Collier county. Samples were collected for both RNA and DNA extractions. Most of the trees are from the PB 56-2 clone but some groves had trees of the older PB 1-2-3 clone. All growers reported lower to no fruit drop on the parson brown irrespective of rootstock used. This is in comparison with Hamlin which had severe fruit drop this year. Hamlin leaf samples from the same block or neighboring blocks were also collected for analysis. 2. Please state what work is anticipated for next quarter:In the next quarter, we will again sample the same groves and collect similar leaf samples. HLB levels will be evaluated using qPCR and we will evaluate the genetic differences between the samples. 3. Please state budget status (underspend or overspend, and why):We are on target with the spending for this project.