1. Please state project objectives and what work was done this quarter to address them: 1. A repetition of the experiment was conducted in late January through February over a period of five weeks. The protocol established is as follows:a. Selected trees were scouted for flush or pruned to induce flushing.b. Areas of flush were bagged and inoculated with 20 parent ACP.c. Parent ACP were removed from bagged flush after two weeks.d. First generation ACP were counted two weeks after removal of parent ACP.e. The presence of native ACP life stages also documented.2. In March all sentinel trees were sampled and tested using gel electrophoresis and rtPCR to confirm the absence of CTVvv-RNAi per permit requirements. Sentinels were also tested via qPCR for the presence of HLB.3. Trial trees were selected for the next repetition of the experiment. These trees were also tested using gel electrophoresis and rtPCR to confirm the presence of CTVvv-RNAi, and qPCR for the presence of HLB.4. Aphid scouting continues on a biweekly basis. The presence of brown aphid has not been detected. 2. Please state what work is anticipated for next quarter: The project will terminate and a final report will be issued 3. Please state budget status (underspend or overspend, and why): On budget