Vismax(R):A novel peptide-based therapeutic for mitigation of citrus diseases, including HLB

Vismax(R):A novel peptide-based therapeutic for mitigation of citrus diseases, including HLB

Report Date: 06/15/2023
Project: 20-015   Year: 2023
Category: Other
Author: Michelle Leslie
Sponsor: Citrus Research and Development Foundation

1. Please state project objectives and what work was done this quarter to address them: Objective: Determine whether Vismax treatment promotes resistance to other [than HLB] major citrusdiseases, specifically Phytophthora root rot and citrus canker. Demonstrate that Vismax treatment of greenhouse-inoculated trees reduces disease severity and/or incidence. Work: Dr. Megan Dewdney (U. Florida IFAS CREC) and her team have been preparing for inoculated Phytophthora root rot greenhouse cone-tainer and Rhizotron trials, in order to evaluate rates of drench-applied Vismax in combination with and comparison to Foliar-applied Vismax for their ability to promote resistance to phytophthora root rot in susceptible orange seedlings by comparing dry structural and fibrous root masses, scoring roots and foliage for visible evidence of Phytophthora infection, and measuring Phytophthora incidence at root tips. 2. Please state what work is anticipated for next quarter: In Q3 2023, with Dr. Dewdney, we plan to execute Phytophthora Root Rot Trials, to expand on the results of the study conducted previously.  The Rhizotron trial is a more detailed and labor-intensive trial that enables root observaton throughout the duration of the trial, giving more detailed data about the timing and level of protection provided throughout disease progression. Additionally, with Dr. Dewdney, we will execute a citrus canker trial, this time focusing on the effect of differential application timings, rates, and application method combinations (e.g. root drench + foliar).  The number and severity of lesions will be scored and imaged for this trial. 3. Please state budget status (underspend or overspend, and why): The project is currently under budget, due to the seasonality of greenhouse disease trials and availability of “clean”, uninfected seedlings for trials. To date, we have used 57% of the allocated funds.  The continuity of collaborator in the past year has allowed us to plan farther in advance, and enable our collaborator to plan more experiments to a tighter timeline. This will enable to us to progress the project at a faster rate in Year 3 and complete objectives for assessing Vismax efficacy for control of citrus canker and Phytophthora root rot.


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