Our project aims to provide durable long term resistance to Diaprepes using a plant based insecticidal transgene approach. In this quarter, all transgenic lines as described in the project proposal have been regenerated and most plants have been acclimatized and transferred into the greenhouse for further growth. A few putative transgenic lines have been transferred to fresh in vitro rooting medium in efforts to stimulate root production. Root samples from 21 lines have been analyzed for gene expression using qPCR. Of them, 12 were determined to be high expressers while the rest were medium to low in expression. We are in the process of evaluating the remaining greenhouse acclimated lines for gene expression. The high expresser lines will be propagated for subsequent evaluation with Diaprepes neonates.