We proposed to identify and assess gene sequences for their negative effects on sap-sucking Hemipteran insects via RNAi using both in vitro and in planta dsRNA feeding assays. Objective 1 of our proposal intended to evaluate candidate genes for dsRNA-induced lethality of Diaphorina citri and our model organism, Myzus persicae, using artificial feeding assays. To date, we have cloned sequences at least 400 bp in length from nine homologous D. citri and M. persicae transcripts. In addition, we have carried out artificial feeding assays on M. persicae using dsRNA derived from the salivary gland-specific Coo2, midgut-specific glutathione-S-transferase S1 (GSTS1) and constitutively expressed S4e ribosomal protein from M. persicae, as well as a control derived from green fluorescent protein (GFP) sequence. While our results suggest that the M. persicae-specific dsRNA has a negative effect on both the lifespan of the insects and the number of offspring generated, additional independent assays are needed to enhance treatment replication to better resolve statistically significant differences among dsRNA treatments. Since the annual report, we have nearly completed our evaluation of the RNAi strategy in planta for its effects against our model insect, M. persicae (objective 2). Since recent evidence suggests that RNAi in sap-sucking insects may operate more effectively in planta than in vitro, this approach may prove to be critical to the success of this study. This research requires the use of Gateway-based vectors that express the selected insect dsRNA either constitutively (35S promoter) or in a phloem-specific manner. We have cloned and characterized two SUS1 promoter alleles (CsSUS1-1 and 2) from Citrus sinensis cv. valencia and have found them to drive phloem-specific expression in both Arabidopsis and tobacco when fused to a reporter gene. Deletion analyses have shown that two separate phloem-specific enhancer regions (nucleotides -1153 to -462, and -410 to -268) exist in CsSUS1-1. Conversely, only the latter enhancer region promotes phloem-specific expression in CsSUS1-2, while the former region drives constitutive expression. We have also successfully generated Gateway vectors that will result in the constitutive (35S promoter) or phloem-specific (CsSUS1 promoter) expression, respectively, of M. persicae-specific Coo2, GSTS1 and S4e dsRNA, as well as a control derived from GFP. In the fall, we began working on objective 3: to transform citrus with RNAi-inducing transgenes against D. citri. Previously, we conducted 3′ rapid amplification of cDNA from vacuolar ATP synthase subunit G, S4e and .-tubulin transcripts from D. citri. We have now inserted sequences of the aforementioned transcripts into Gateway-based vectors downstream of both the constitutive 35S and our novel phloem-specific citrus CsSUS1 promoters. In the New Year, we will begin transforming citrus with the D. citri-specific gateway vectors for use in subsequent feeding assays with D. citri by Florida collaborators. Initial attempts at citrus transformation at Cornell suggest that Citrus sinensis cvs. Valencia and Hamlin can be transformed and regenerated. In summary, we have cloned a number of transcripts from both D. citri and our model organism, M. persicae, and have analyzed a subset of derived dsRNAs to test their effect on M. persicae using in vitro assays (objective 1). We have also cloned and characterized several novel phloem-specific promoters from C. sinensis, and have evaluated their expression patterns. We also created new Gateway-derived vectors bearing a native citrus phloem-specific promoter, for use in RNAi in our M. persicae model and evaluated them in planta (objective 2). Finally, we’ve now generated similar vectors specifically designed against D. citri (objective 3), and preparing to put them into citrus with our developing citrus transformation infrastructure.
This project is being terminated early due to departure of the post-doc working on the objectives. Certain objectives, including the work investigating the basis of resistance in Cleopatra mandarin will be continued as objectives of other grant proposals. Following this report, no more funds will be spent from this project and should be considered closed out. To summarize the results of the past two years work on this project: We examined the fitness of ACP on various citrus varieties to determine whether ACP populations might be higher on certain varieties. For example, previous reports in the literature suggested that grapefruit was a better host than most sweet orange varieties. In our studies, we found little meaningful difference in suitability of the commonly used citrus cultivars for ACP; in fact, grapefruit appeared to be less acceptable than sweet orange varieties. We also found that the potential for induced host preference did not appear to exist for ACP. Oviposition and survival rate of ACP reared on rutaceous plants in one genus and transferring the F1 generation to a rutaceous plant of an entirely different genus had no effect. One citrus root stock material that we examined, Cleopatra mandarin, was found to be resistant to ACP. Adult psyllids were found to lay eggs on Cleopatra mandarin plants, but upon eclosion, the nymphs failed to complete development to the adult stage. Evaluations of a series of Cleopatra mandarin hybrids identified a number of plants which varied in their susceptibility to ACP. Electrical penetration graph studies investigating the feeding behavior of psyllids on these plants has begun in attempts to help identify the mechanism for this host plant resistance. EPG recording and analysis of psyllid feeding on Cleopatra hybrids will continue in future planned grant proposals. The effect of host plant nutritional status was also examined. In particular, the effects of Nitrogen, Potassium and Boron were found to have the most influence on psyllid fitness. Similar to the results found in other piercing sucking insects such as aphids, increased levels of nitrogen resulted in higher reproductive rate, a shorter development time and overall larger body mass per individual. On the other hand, potassium significantly reduced both reproductive and growth rates. increased rates of Boron resulted in high rates of mortality of ACP. EPG studies of ACP on Boron treated plants altered certain aspects of psyllid feeding behavior.
Progress on a new special regulatory trap for capturing and preserving citrus psyllids in situ has been advanced enough to develop some prototypes for further field testing and refinement. Laboratory bioassays that examined psyllid behavior in response to physical details on the surface of the traps is ongoing. Our premise is that eventually some types of attractants will be identified and deployed with trap visual cues optimized in yellow to enhance the numbers responding to the vicinity of traps. However, the trap capture efficiency will depend on the psyllid behavior on the trap relative to their willingness to orient, land and enter the trap. We are concentrating on testing and improving trap components to optimize these behaviors.
In a previous report, we showed that acquisition efficiency of Ca. Liberibacter asiaticus (Las) by Diaphorina citri was related to the duration of acquisition access periods (AAP), increasing with time available for feeding on infected plants. In this report, we describe the relationship between duration of AAP and transmission efficiency by nymphs and adults. Groups of 30 psyllid adults (1-wk old) or 3rd-4th instar nymphs were confined on leaves of a young shoot of a symptomatic infected plant, with recently expanded leaves, inside sleeve cages, for AAPs of 1.5, 6, 12, 24, 48 or 96 h. After the AAP, the insects of each group were first transferred to healthy citrus seedlings in order to complete a latent period of 14 days at 25C, and then transferred to healthy test seedlings (5 insects/plant) for two consecutive 7-day inoculation access periods (IAP). After the second IAP, total DNA of each insect (sample of 10-20 insects per AAP treatment) was extracted and submitted to qPCR for detection of Ca. L. asiaticus. The experiment was repeated three times, using different source plants of the pathogen for the AAPs. Transmission efficiency of Las by D. citri nymphs and adults, as measured by the proportion of test plants that became infected after exposure to the psyllids during the two consecutive 7-day IAPs, also increased with AAP duration. Las transmission rates increased from 14 to 33% when nymphs were submitted to AAPs ranging from 1.5 to 48 h. In the case of Las acquisition by adults, transmission to test plants was first detected when the insects were allowed a 6-h AAP, reaching a maximum of 19% for an AAP duration of 48 h. Considering that the test plants were inoculated by groups of five psyllids, we used the method proposed by Swallow (1985) to estimate the transmission probabilities by single vectors (P), by using the formula, P = 1-(1-p)1/k , in which p is the proportion of infected test plants and k is the number of individuals used to inoculate each test plant. The transmission probabilities by single psyllids after an AAP of 48 h by nymphs and adults were estimated in 0,077 (or 7.7%) and 0,042 (or 4.2%), respectively. These transmission probabilities are rather low, considering that mean acquisition rates after a 48-h AAP by nymphs and adults were around 90% and 60%, respectively. The occurrence of transmission rates >10 times lower that the acquisition rates strongly suggest the existence of a selective barrier within the psyllid body that may be affecting the passage of the bacterial cells to internal organs involved in inoculation of the pathogen. According to Ammar et al. (2011), a lower proportion of salivary glands were colonized by Las cells in relation to other organs in infective psyllids, suggesting that the passage to the salivary gland may be an important barrier involved in Las transmission. Because significant proportions of nymphs and adults were able to acquire and transmit Las after relatively short AAPs (1.5 and 6 h, respectively), we conduct an additional experiment to evaluate the possibility of bacterium acquisition with AAPs shorter than 1.5 h. Groups of 30 psyllid adults (1-wk old) or third-instar nymphs were confined on leaves of a young shoot of a symptomatic infected plant, with recently expanded leaves, inside sleeve cages, for AAPs of 15, 30, 45, 60 and 90 min. After the AAP, the insects of each group were first transferred to healthy citrus seedlings in order to complete a latent period of 14 days at 25C, and then transferred to healthy test seedlings (5 insects/plant) for two consecutive 7-day inoculation access periods (IAP). After the second IAP, total DNA of each insect (sample of 15 insects per AAP treatment) was extracted and submitted to qPCR for detection of Ca. L. asiaticus. The qPCR results showed that nymphs and adults successfully acquired the pathogen after AAPs ’30 min. No acquisition was observed during the shortest PAA tested (15 min), suggesting that >15 min of psyllid exposure to infected plants is necessary for stylet penetration and contact with infected tissues for successful acquisition of Las.
Report: ACP dwelling in rutaceous plants outside of commercial citrus orchards are a source of HLB. If not controlled, these ACP will stymie the effectiveness of area-wide management programs aimed at containing the spread of HLB in commercial citrus. The proposed research is to develop an autodisseminator (‘dispenser’) of pathogen spores for controlling ACP in residential and organic citrus. The working hypothesis is that ACP will visit the dispenser, pick-up spores, and infect other psyllids when they return to the foliage. The dispenser will be coated with spores of the fungus Isaria fumosorosea (Ifr), a known pathogen of ACP, and will be designed to attract and retain psyllids and to efficiently infect them with spores. Greenhouse tests will determine if infected psyllids can transmit the pathogen to immatures and adult cohorts. Tests will be conducted in local communities to determine if the dispenser can effectively infect ACP in residential trees and to assess spore viability over time. Information from these tests will be used to optimize ACP attraction to the dispenser and spore viability and transfer. The ultimate goal is to design a dispenser that is effective and safe, acceptable to regulatory agencies, and readily adopted by homeowners and organic growers. Exposure of free-fly ACP in a greenhouse resulted in an average of 55% of the ACP becoming infected with pathogen spores (n = 3 trials), demonstrating that the proposed concept is very plausible. The dispenser used for greenhouse tests is constructed from a 21.25 cm x 27.5 cm (8.5 in. x 11 in.) sheet of bright yellow stock paper rolled into a tube with eight pleated ridges running lengthwise across its surface. ACP prefers to crawl along edges and the ridges increase ACP retention on the device, in turn increasing the likelihood of spore infection. The inner portion of each pleat is coated with a thin line of SPLAT (ISCA Technologies, Inc.), an emulsified wax used to dispense scent compounds. The SPLAT contains a standardize aliquot (10’l scent mixture per 10 ml SPLAT) of a terpene mixture based on the emission of volatiles from growing terminal shoots of ACP host plants. Ongoing tests are showing that scented SPLAT induces significantly more probing by ACP than unscented SPLAT. Greenhouse tests will be soon be initiated to determine whether nymphs can be infected from adults carrying Ifr blastospores.
Field trials were conducted in fall 2010 to determine how best to apply systemic insecticides to young trees to gain the longest lasting duration of psyllid control possible. Three neonicotinoid insecticides were evaluated in replicated field plots on trees approximately two years of age. These three products were imidacloprid (Admire), thiamethoxam (Platinum) and clothianidin (a product expected to be registered for use in citrus in the coming year). Each of these products were evaluated using three different application methods; soil drench, soil band spray and trunk application with multiple application rates for each method tested. For the trunk applications with imidacloprid, in addition to evaluating Admire, trunk applications were also made using Confidor 200SL which is the formulation reportedly used in the past for trunk applications in South Africa. The results from our first trial showed that soil-band applications (at the rates and volumes applied) were the least effective method for applying neonics for systemic protection of young trees. The traditional soil-drench application and trunk application appeared to provide the most consistent results in terms of psyllid control. Because of the low psyllid populations present, we were not able to determine whether trunk applications made using different rates provided better control than soil drench applications in this trial. We did however show that our available imidacloprid formulations were equal to, if not more effective than the product (Confidor) used in South Africa that has been touted as providing months of residual control of African psyllids. Work is continuing this spring to further investigate the most effective rate and application method of these products on trees of varying sizes. Work was also initiated in fall 2010 to determine the effect of combining psyllid repellents with soil-applied neonicotinoids to further reduce the likelihood that young trees will become infected with the HLB pathogen before reaching bearing age. These studies have been successful in determining the minimum dose required to deter psyllid feeding and we are currently doing residue studies to determine the effect of rainfall on duration of efficacy.
Imidacloprid is a systemic insecticide that is applied to the soil through irrigation systems. Imidacloprid follows the water-conducting channels (xylem) within the tree and is thus easily distributed to all tissues. Many insects, including the Asian citrus psyllid (ACP), feed from the vascular system. Systemic pesticides like imidacloprid are, therefore, ideal against such pests since they directly target the insects during feeding. One of the attractive features of imidacloprid is its persistence. A good application can result in several months of protection. And, because of its systemic properties, imidacloprid will move from older tissues into developing leaf tissues during flushes. In our research we are studying the conditions within citrus groves that are conducive to better imidacloprid uptake. At a commercial grapefruit grove in Riverside County, we evaluated 2 application rates (1X label rate and an experimental 2X label rate) of imidacloprid in 50-year old trees growing on sandy soil. Late Summer/early Fall applications of imidacloprid were very effective with rapid uptake and persistence through the winter months to the following Spring. At the higher experimental 2X rate, the imidacloprid concentrations in young flush recovered to levels that would be toxic to ACP. With early Spring applications, there was a long delay before the appearance of imidacloprid within the leaf tissue at ACP target threshold levels. The experimental 2X rate was noticeably more effective than the 1X rate, providing good protection to trees at a time that is likely to be critical for ACP control should the insect establish on commercial citrus in California. The delay in uptake observed with the Spring applications may be linked to poor root development at that time of year. Applications later in the year occur at a time when root activity is well established, thereby allowing for a more rapid rate of uptake. At a second site in Ventura County, 24-year old lemon trees growing on a heavy clay soil were treated with the label rate of imidacloprid. Treatments between June and August resulted in very poor uptake and on no occasion during the season did imidacloprid concentrations reach the ACP threshold level. Interestingly, imidacloprid levels within the trees appeared to spike at several times during the season, albeit at low levels. This pattern is likely due to the irrigation schedule used at this site, which is on a 3-week cycle. However, with long periods between irrigations, imidacloprid uptake was less effective. The impact of irrigation frequency on imidacloprid uptake was compounded by the heavy soil conditions at the lemon grove. Imidacloprid can become bound up in heavy clay soils, particularly when water is not available to keep the imidacloprid solubilized. With no water to release it from the binding sites on soil particles, imidacloprid uptake may be compromised. At the grapefruit grove, irrigations were more frequent (weekly) and, combined with the sandier soil conditions, there was less opportunity for imidacloprid to become bound up and unavailable for uptake. Comparisons of imidacloprid behavior in citrus groves are critical to our understanding of the role that imidacloprid will play in the management of ACP.
By law in California, citrus nursery stock must be treated with both a systemic and foliar pesticide in order to move it into areas quarantined for the newly arrived pest, Asian citrus psyllid (ACP). The purpose of these treatments is to prevent ACP from establishing in nurseries and being moved around California when the plants are sold. Imidacloprid is one of the systemic insecticide options available to nurserymen and is the focus of our research at UC Riverside. The California Department of Food and Agriculture (CDFA) typically requires that the maximum label rate of the systemic product be used before allowing movement of regulated nursery stock within the ACP quarantine area for a 3-month period. At the time we submitted our original research proposal in 2009, efforts were underway by Bayer CropScience to raise the maximum label rate of Admire Pro’ for ACP control from 0.33 ml/cu ft to 5 ml/cu ft potting media, a 15-fold increase. Although that label was approved, the CDFA is not enforcing the use of the new maximum label rate until data are available supporting the need for such a comprehensive increase. Our trials are designed to evaluate different application rates, as well as several factors that might impact the uptake of imidacloprid into potted citrus, and the data generated will then be used in the decision-making processes concerning suitable rates of application. Higher rates of Admire Pro’ application resulted in higher levels of imidacloprid within the leaf tissue. At application rates we tested (ranging from 0.33 ml to 5 ml/cu ft potting media), we observed no saturation of imidacloprid levels when rates were increased. On 2-year old containerized trees, the maximum allowable rate under the label of 5 ml/cu ft potting media would seem to be excessive because the concentrations of insecticide within the foliage were in vast excess of that which is required for management of ACP. We are currently evaluating additional rates of application intermediate between the 0.33 ml and 5.0 ml rates. Additional information is required to determine whether there is a rootstock effect on imidacloprid uptake. The data we have generated thus far suggest that there is an effect; we observed differences in uptake between Minneola Tangelos budded on Carrizo and Macrophylla, with more rapid uptake in the Macrophylla trees. Monitoring data from a commercial nursery showed higher concentrations of imidacloprid in Valencia trees budded on C35 compared with similarly aged trees on Trifoliate. Monitoring imidacloprid levels in nursery stock is an important exercise if we are to optimize imidacloprid use for containerized citrus. The ACP targets the youngest flush on trees for feeding and oviposition, so it is imperative that young flush is protected. Protecting the young flush is probably the biggest challenge for imidacloprid because there may be a lag-time between the emergence of new flush and the establishment of imidacloprid within that tissue. For this reason, the foliar treatments are critical to provide the protection to the flush until the imidacloprid becomes established at toxic concentrations.
Transmission of Ca Liberibacter asiaticus (CLas) by the Asian citrus psyllid (ACP) involves complex psyllid-plant-pathogen interactions, which should be understood in order to improve management strategies to control Huanglongbing (HLB). The goal of this research is to determine factors that influence the risks of acquisition or inoculation of the pathogen (CLas) by ACP, e.g vector developmental stage, feeding periods, leaf age and symptom expression/bacterial population in disease plants, in order to optimize strategies to avoid or reduce disease spread within and between citrus groves. In study 1, we first compared acquisition efficiency of CLas by different ACP nymphal stadia (1st, 2nd, 3rd, 4th and 5th instars) and adults (1 wk old) after a 48-h acquisition access period (AAP) on infected plants. Mean acquisition efficiencies by adults and 1st, 2nd, 3rd, 4th and 5th instars, as measured by the proportion of infective psyllids by RT-PCR, were 42.9, 68.4, 60, 66.6, 84 and 65.3%, respectively. CLas concentration reached higher levels in ACP when acquired by 4th and 5th instar nymphs. In a second experiment, we compared CLas acquisition efficiency by 3rd-instar nymphs and adults with respect to duration of the AAP (1.5, 6, 12, 24, 48 or 96 h). Almost 30% of the nymphs and adults were PCR positive for CLas when submitted to the shortest AAP tested, suggesting that the minimum AAP required for acquisition is <1.5 h. Nymphs and adults efficiently acquired the pathogen (>90% infective psyllids) when allowed to feed for at least 1 and 4 days, respectively, on young shoots of infected plants. However, transmission rates to test plants did not change significantly with increasing AAPs, reaching a maximum of 9.4 and 4.8% (per individual) with a 12-h AAP by nymphs and adults, respectively. By comparing electrical penetration graphs (EPG) of nymphs and adults , we found no significant variations in feeding behavior that could account for differences in acquisition and transmission efficiency between the two development stages. Mean time to start phloem sap ingestion (waveform E2) was 63 and 75 min for nymphs and adults, respectively . This explains why a significant proportion of insects can acquire the phloem-limited CLas with a 1.5-h AAP. In study 2 we investigated the effect of leaf age (young and asymptomatic vs. symptomatic mature leaves) in CLas source plants on pathogen acquisition and vector probing behavior, showing that more efficient acquisition depends on the availability of young leaves in infected plants, apparently because phloem ingestion by ACP adults is more frequent and last longer on the younger leaves (see previous reports). In study 3 we evaluated CLas acquisition efficiency by ACP adults on infected citrus with variable pathogen titers, and found that acquisition can occur on asymptomatic infected plants, but efficiency is higher on plants with higher bacterial titers, usually symptomatic (see previous reports). In study 4, we are studying the relationship between duration of the inoculation access period (IAP) and transmission efficiency of CLas by ACP. A first experiment was conducted to determine when insects start inoculating the pathogen after acquiring it from source plants (latent period), and the results show that the latent period is around 10 days. So, we set up 4 trials of a second experiment where the insects were confined for a 2-week AAP on infected plants and then submitted to variable IAPs (0.5, 1.5, 6, 12, 48, 24) on test plants. A third experiment was set up to determine EPG waveforms related to inoculation and minimum IAP to perform such waveforms. In Study 5, we will be testing if systemic and contact insecticides can prevent CLas inoculation on plants with or without young shoots. Results of studies 4 (2nd and 3rd experiments) and 5 will be available during the third and last year of this project.
Asian citrus psyllid (ACP), Diaphornia citri Kuwayama (Hemiptera: Psyllidae), was detected in Southern California in August 2008. ACP is a serious citrus pest because of its ability to acquire and vector a lethal plant pathogenic bacterium Candidatus Liberibacter asiaticus which causes a lethal disease called Huanglongbing (HLB) or citrus greening. The bacterial pathogen of HLB has not yet been detected in California, but is widely distributed in Florida, Georgia and various states of Mexico. The California Department of Food and Agriculture (CDFA) is monitoring ACP and control programs are focused on containing ACP through localized eradication attempts with insecticides. Insecticide applications, however, are not acceptable in organic citrus orchards and may not be desirable in residential urban areas. Biological control of ACP maybe an acceptable alternative in these situations while providing an additional weapon for control of ACP in commercial production systems. Tamarixia radiata is the most commonly used ACP parasitoid. A strain of T. radiata from Pakistan is of primary interest for the California citrus industry due to a good climatic match between citrus growing areas in California and Punjab region of Pakistan. Before Tamarixia can be released for ACP control in California, it is mandatory to complete an Environment Assessment Report assessing the threat that the introduction of a new living organism may present to California, especially to native non-target psyllid species. Attacks by Tamarixia on non-target native psyllids could unduly threaten the natural balance between native psyllids with their native host plants and the native natural enemies of those psyllids. Additionally, attacks on beneficial psyllids, such as Arytainilla spartiophylla, a biocontrol agent of Scotch broom, a highly invasive weed, may reduce biological control of this weed. To address this regulatory concern, representative non-target psyllids have been selected for host specificity testing based on: (a) native California psyllids that are phylogenetically related to ACP, (b) native California psyllids with higher probability of occurrence around citrus groves because they are found on common native California plants that inhabit wilderness areas around citrus orchards, (c) native California pest psyllids, (d) introduced pest psyllids, and (e) imported weed biological control agents. Some of the identified non-target native psyllids have been collected and are being reared on their respective host plants. An ACP and Tamarixia colony are being maintained in quarantine at UC Riverside. For host-specificity testing, non-target psyllid species on their respective host plants are being presented to female Tamarixia under choice and no choice conditions to determine the attack preferences of this natural enemy. safety tests for three native psyllid species have been completed; Heteropsylla sp. on Acacia farnesiana, Heteropsylla texana on Prosopis glandulosa, and Calophya californica on Rhus ovata. None of these native psyllids were parasitized by Tamarixia. Other psyllids from host plants such as Manzanita, flannel bush, potato, olive and scotch broom will be tested for suitability as hosts for Tamarixia as they become available.
This project provides funding for monitoring for pesticide resistance in key pests of San Joaquin Valley California citrus, screening new pesticides for efficacy against pests and selectivity favoring natural enemies, and pheromone trapping and decision making web applications. Pesticide resistance monitoring of California red scale using a colorimetric assay that measures esterase enzyme levels continues to document resistance to organophosphates and carbamate insecticides in numerous populations. Resistance monitoring of California red scale using a fruit dip bioassay has occasionally detected low levels of resistance to the insect growth regulator pyriproxyfen (Esteem), which has been the most commonly used insecticide for red scale control for the past 12 years. Work continues to determine the best application methods and timing for spirotetramat (Movento) and several new compounds to aid in resistance management. Pheromone trapping is conducted for California red scale and posted on the www.ucanr.org/sites/KACCitrusentomology/ web site to assist PCAs in timing of applications. Pesticide resistance monitoring of citricola scale using a leaf dip bioassay documented resistance to the organophosphate chlorpyrifos in numerous orchards. Resistance has reduced the residual efficacy of this pesticide from 3-5 years to 1-2 years, causing citricola scale to become the number one pest in the San Joaquin Valley. Pesticide screening indicates that foliar neonicotinoids provide 1-2 years of citricola scale control, however, they are broad spectrum and disrupt natural enemies needed for other pests. Screening is underway to find additional chemical groups with activity against citricola scale both to reduce the frequency of applications and to manage pesticide resistance. Pheromone disruption of citrus leafminer is being studied in greenhouses. Pesticide screening for citrus leafminer indicates that many pesticide groups provide short-term (one flush) control of leafminer. A few insecticides, notably abamectin, spinetoram, and rynazypyr provide longer control. Results of this project are presented in Arthropod Management Tests, field days, citrus industry meetings and Citrograph. A treatment program for Asian citrus psyllid in various growing regions of California has been developed based on Florida and Texas research and knowledge of the IPM program in California.
An oil treatment program for Asian citrus psyllid is proposed for situations such as organic blocks where conventional insecticides can not be used. The objective of the project is to determine the effects of 0, 6 (fall) and 12 (spring and fall) applications of PureSpray green 0.25% oil applied in 250 gpa using an airblast sprayer with outside coverage, on tree health, fruit production and fruit quality of California navels. The experiment is being conducted at Lindcove Research and Extension Center in Exeter CA. The plots (5 rows by 8 trees) were assigned treatments based on pretreatment evaluations of fruit yield and size prior to treatment. Treatments were applied to three replicated plots during spring (6 applications 2 weeks apart) and fall (6 applications 2 weeks apart) during 2010. Trees were evaluated for leaf drop and no significant differences were found between treated and nontreated trees. In November 2010, color was evaluated in the four quadrants of 6 trees per plot. The percentage of fruit with 75-100% color and juice weight were significantly higher in the two oil treatments compared to the nontreated control, indicating the oil treatments affect fruit maturation. Fruit quality measurements (brix, California Standard, rind thickness, juice weight, fruit length and width) are currently underway. Harvest of individual trees will be conducted in February 2011 and we will evaluate yield, fruit size and fruit grade. This experiment will determine if repeated low rate, low volume oil applications affect citrus production or fruit quality under California conditions. This information is critical as we prepare for the spread of Asian citrus psyllid into commercial citrus orchards.
Evaluation of the passage and mortality Diaphorina citri on screens impregnated with insecticide The experiment was conducted in two farms located in S’o Paulo State. The first farm is located in the municipality of S’o Manuel, where a block was selected that had a strong record of psyllids capturing in its surround. The barrier installed, has a length of 100 meters, width of 2.7 m and a distance to the ground about 60 cm. This was positioned next to the orchard at a distance of 5 m. The green sticky traps were equally divided between areas with and without barrier, 15 days after the first assessment was conducted, but none of the areas the psyllid was collected. In the second farm, the experiment was similar to the first one; however in this case an additional protective barrier 80 m was added to the side of the block. On this farm the first evaluation has not been realized. A first selection to determine which screens have the potential to be used as barriers was carried out. D. citri could go through all the screens evaluated (75%). But it was observed that after contact with the screen, about 85% of the insects have died before having contact with citrus trees, except the screen which showed a COD 196 60% mortality. For the field trials was selected to screen COD205. This was sent by the supplier, but with a higher dose (4g deltamethrin/kg of net). A new test was performed, and observed a mortality of 98.3%. Evaluation of screens impregnated with insecticide barriers The experiment was conducted in two farms located in S’o Paulo State. The first farm is located in the municipality of S’o Manuel, where a block was selected that had a strong record of psyllids capturing in its surround. The barrier installed, has a length of 100 meters, width of 2.7 m and a distance to the ground about 60 cm. This was positioned next to the orchard at a distance of 5 m. The green sticky traps were equally divided between areas with and without barrier, 15 days after the first assessment was conducted, but none of the areas the psyllid was collected. In the second farm, the experiment was similar to the first one; however in this case an additional protective barrier 80 m was added to the side of the block. On this farm the first evaluation has not been realized.
Under Objective 1 (define rates and formulations of copper sprays for more effective control) one trial with Red grapefruit was conducted in Ft. Pierce, FL and one trial with Hamlin orange in Hardee County, each with 14 treatments of formulations, rates and combinations with other chemical treatments of interest. This season, trials had low to moderate disease epidemics as a result of a periodically wet spring, moderately wet summer and an absence of tropical storm events. Evaluations of fruit infection in these trials were made in November 2010 (grapefruit) and January 2011 (Hamlin). Copper formulations containing copper hydroxide or basic copper sulfate (metallic rates of 0.67 to 1.12 kg/ha), varied from low (Hamlin) to moderately effective (grapefruit) for canker control of fruit disease incidence. Copper pentahydrate, at a lower metallic copper rate/ha per application, provided equivalent control to film-forming copper formulations. Greater canker susceptibility of fruit occurred in later season and was likely because of more prolonged opening of stomates in cooler weather and enhanced bacterial entry, coincident with more numerous windblown rain events. Although Hamlin fruit disease incidence was higher, copper protection against early season fruit infection was effective for prevention of premature fruit drop. Under Objective 2 (establish the period of fruit susceptibility, residual activity and phytotoxicity of copper). In 2010, we compared the copper fruit residues from different copper-containing products up to 28 days after application to grapefruit. The copper product used and the number of days after application significantly affected the amount of copper residue. These effects were consistent whether the data were analyzed as copper/fruit or copper/surface area. The rate of copper residue decrease was different for the various products tested. Copper products forming films, Kocide 3000 (copper hydroxide), Cuprofix Ultra 40 Disperss (copper sulfate), and Badge X2 (copper hydroxide and oxycloride) decreased at a similar rate, whereas the non-film forming Magna-Bon CS2005 (copper sulfate pentahydrate) decreased at a higher rate and left about 75% less fruit residue. This was expected as Magna-Bon is applied at approximately 15% of the metallic contained in film-forming coppers. Since Magna-Bon performed as well as film-forming copper products for control of canker on grapefruit the last two seasons, we have hypothesized that the Magna-Bon copper may be locally systemic in fruit rind tissues. This coming season, we will attempt to assay the activity of copper in the rind against Xcc to determine whether the copper is located internally as well as externally. Under Objective 3 ( evaluate the use of streptomycin [Firewall]) As in the past three seasons, applications of Firewall in July and August, were effective for canker control on grapefruit either in combination with a reduced rate of copper or when substituted for copper in the spray program. Based on Firewall’s efficacy and ability to lower the risk of copper phytotoxicity, an application has been submitted by FFVA and FDACS to gain EPA Section 18 emergency registration for use of Firewall against canker on fresh grapefruit. Under Objective 4 (To define risk for development of bacterial resistance to copper and streptomycin in FL citrus groves) a number of factors favorable for the development of copper resistance in Xcc were identified. Findings are being prepared for publication. Under Objective 5 (rapid transfer improved canker management technology to the Florida citrus industry), 2011 canker management recommendations have been submitted for publication in the Florida Citrus Pest Management Guide and to Citrus Industry Magazine. Oral presentations have been delivered at the Florida Citrus Show and at a multi-county extension meeting. Results of the Hamlin trials will be presented at the 2011 FSHS annual meeting.
Objective 1 is to conduct a field evaluation nutritional sprays for control of HLB and HLB symptom expression and yield. The field study was set up May 2010 in Southern Grove, Hendry Co., FL. Six treatments were set in 4 plots of 150 trees per treatment (interior 10 trees in each block were identified for PCR, leaf nutrition sampling, tree health and yield evaluation). Treatments were 1) non-treated check; 2) Nutri-Phite sprayed 4 times bimonthly; 3) N-Sure sprayed bimonthly; 4) Agra Sol Mn/Zn/Fe plus Nutri-Phite plus triazone urea sprayed bimonthly; 5) Keyplex 1400 DP plus Nutriphite plus triazone urea sprayed bimonthly; 6) Wettable powder nutrients (Diamond R #2) plus Nutri-Phite P+K sprayed bimonthly. The materials were applied to both sides of the tree in 125 gallons per acre with an airblast sprayer driven at 2 mph to obtain thorough coverage. Two disease ratings have been taken so far and no significant change in tree health has been observed yet in treated or control, including symptomatic trees. There were no significant treatment differences in yield at the first harvest, after the initiation of treatments in April. Objective 2 is to determine the mechanism of HLB symptom suppression by foliar nutritional application, Hamlin sweet orange trees have been inoculated and are being treated bimonthly with the nutritional sprays 1, 2, 3, and 5 from objective 1. Infection rate and progress are being monitored by qPCR monthly. Two months after inoculation, new flush on some trees was strongly positive for Las; however, too few of the trees were positive to determine any treatment effects. Samples from 3 and 4 months post inoculation continue to show an increase in trees detected positive. No significant differences have been observed, although treatment 5 has consistently had the highest number of positive trees each month. Five month qPCR samples showed a leveling off of incidence as most of the trees test positive and the number of trees still testing negative agrees with known graft inoculation efficiencies. Routinely, the first positive sample on a tree is in the newest flush and older leaves remain negative or only weakly positive. No significant differences in Las titer have been observed between treatments to date, although the highest Las titers each month have been in treatment 3 and 5. Initial positives were detected in new flush near the graft inoculation site. 5 months post inoculation Las appears to be moving systemically through the canopy with detections occurring in old leaves, recently hardened off leaves, and topmost new flush depending on the plant. There is a trend towards high incidence of detection in the topmost flush of treatment 5, which, combined with higher titers, could have implications for vector transmission in disease management. Sampling and fixing of plant material for microscopy of phloem and leaf blade tissue began at two months and continues monthly. Fixed samples will be observed by microscopy once strongly symptomatic tissue is available for comparison. At five months, symptoms began to develop on tissues that had been Las positive the longest according to qPCR. This suggests that it takes 2-3 months after Las invades leaf tissue for symptoms to develop. No treatment differences in symptom development have been observed, except that symptom development first began in the trees from treatment 3 and 5 that were the first to test positive 2 months after inoculation. Currently, no differences in tree vigor have been observed, but this is not expected until strong symptoms develop. As soon as symptoms develop in control plants, analysis of fixed samples will begin, which is expected at month 6 or 7. Sampling for 6 months post inoculation is underway at the time of this report.
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