Plant Improvement


Quarterly Project # 21-025: Transgenic capable field site to assess HLB-resistant and other improved citrus

Report Date: 05/06/2026   Project: Project # 21-025   Year: 2025

Quarterly Project # 21-025: Transgenic capable field site to assess HLB-resistant and other improved citrus

Report Date: 05/06/2026
Project: Project # 21-025   Year: 2025
Category: Plant Improvement
Author: Matthew Mattia
Sponsor: Citrus Research and Development Foundation



Quarterly Project # 21-025: Transgenic capable field site to assess HLB-resistant and other improved citrus

Report Date: 05/06/2026   Project: Project # 21-025   Year: 2026

Quarterly Project # 21-025: Transgenic capable field site to assess HLB-resistant and other improved citrus

Report Date: 05/06/2026
Project: Project # 21-025   Year: 2026
Category: Plant Improvement
Author: Matthew Mattia
Sponsor: Citrus Research and Development Foundation



Quarterly Project # 21-025: Transgenic capable field site to assess HLB-resistant and other improved citrus

Report Date: 05/06/2026   Project: Project # 21-025   Year: 2026

Quarterly Project # 21-025: Transgenic capable field site to assess HLB-resistant and other improved citrus

Report Date: 05/06/2026
Project: Project # 21-025   Year: 2026
Category: Plant Improvement
Author: Matthew Mattia
Sponsor: Citrus Research and Development Foundation



Evaluate new transgenic rootstocks for HLB tolerance

Report Date: 04/13/2026   Project: 23-030   Year: 2026

Evaluate new transgenic rootstocks for HLB tolerance

Report Date: 04/13/2026
Project: 23-030   Year: 2026
Category: Plant Improvement
Author: Zhonglin Mou
Sponsor: Citrus Research and Development Foundation



Evaluate new transgenic rootstocks for HLB tolerance

Report Date: 04/01/2026   Project: 23-030   Year: 2025

Evaluate new transgenic rootstocks for HLB tolerance

Report Date: 04/01/2026
Project: 23-030   Year: 2025
Category: Plant Improvement
Author: Zhonglin Mou
Sponsor: Citrus Research and Development Foundation



Evaluate new transgenic rootstocks for HLB tolerance

Report Date: 04/01/2026   Project: 23-030   Year: 2025

Evaluate new transgenic rootstocks for HLB tolerance

Report Date: 04/01/2026
Project: 23-030   Year: 2025
Category: Plant Improvement
Author: Zhonglin Mou
Sponsor: Citrus Research and Development Foundation



Consolidation of citrus breeding plant material to vacate space for Stage I and Stage II field trials and to exploit tolerant germplasm for gene editing strategies

Report Date: 02/27/2026   Project: 23-029   Year: 2025

Consolidation of citrus breeding plant material to vacate space for Stage I and Stage II field trials and to exploit tolerant germplasm for gene editing strategies

Report Date: 02/27/2026
Project: 23-029   Year: 2025
Category: Plant Improvement
Author: John Chater
Sponsor: Citrus Research and Development Foundation



Consolidation of citrus breeding plant material to vacate space for Stage I and Stage II field trials and to exploit tolerant germplasm for gene editing strategies

Report Date: 02/27/2026   Project: 23-029   Year: 2025

Consolidation of citrus breeding plant material to vacate space for Stage I and Stage II field trials and to exploit tolerant germplasm for gene editing strategies

Report Date: 02/27/2026
Project: 23-029   Year: 2025
Category: Plant Improvement
Author: John Chater
Sponsor: Citrus Research and Development Foundation

1. Please state project objectives and what work was done this quarter to address them:

Objective 1: Destroy declining plant material with no direct use to the citrus industry to make space for Stage I and Stage II trials (Years 1 and 2).

Space created from germplasm removal activities have made tree spaces for more trials and other plantings. Many tree spaces that were made available from grove removal activties have been replanted with trails or promising accessions from the breeding program. Trials that have been planted previously are under management, some of which are from other projects, including a project with trials designed by the Coca Cola Company. More material is slated to be pushed during this final quarter to make even more room for trials and other promising breeding material. The total citrus acreage pushed and/or abandoned and slated to be pushed during this project reporting period is 15.1 acres.

Objective 2: Conserve, move, consolidate, and propagate tolerant and resistant citrus trees to a centralized location. (Years 1 and 2).

Trees are ready to plant in spring 2026 after the last threat of freeze (in March). The planting of these trees is the final significant activity of this project. Trees have been spaded and after the threat of freeze, more trees will be spaded. We will propose to purchase a larger spade to collect the largest, healthiest specimens for consolidation because the USDA spade is not useful for moving the largest HLB tolerant specimens.

2. Please state what work is anticipated for next quarter:

Tree spading and removal of additional material for space for more Stage II trials. Planting of propagated trees in a consolidated block at the CREC.

3. Please state budget status (underspend or overspend, and why):

The budget is just a little overspent due to the no-cost extension, but is largely on track (86% time spent versus 94% funding expended). We are set to have expended all or nearly all funds in an efficient manner.



Consolidation of citrus breeding plant material to vacate space for Stage I and Stage II field trials and to exploit tolerant germplasm for gene editing strategies

Report Date: 02/27/2026   Project: 23-029   Year: 2025

Consolidation of citrus breeding plant material to vacate space for Stage I and Stage II field trials and to exploit tolerant germplasm for gene editing strategies

Report Date: 02/27/2026
Project: 23-029   Year: 2025
Category: Plant Improvement
Author: John Chater
Sponsor: Citrus Research and Development Foundation

1. Please state project objectives and what work was done this quarter to address them:

Objective 1: Destroy declining plant material with no direct use to the citrus industry to make space for Stage I and Stage II trials (Years 1 and 2).

Space created from germplasm removal activities have made tree spaces for more trials and other plantings. Many tree spaces that were made available from grove removal activties have been replanted with trails or promising accessions from the breeding program. Trials that have been planted previously are under management, some of which are from other projects, including a project with trials designed by the Coca Cola Company. More material is slated to be pushed during this final quarter to make even more room for trials and other promising breeding material. The total citrus acreage pushed and/or abandoned and slated to be pushed during this project reporting period is 15.1 acres.

Objective 2: Conserve, move, consolidate, and propagate tolerant and resistant citrus trees to a centralized location. (Years 1 and 2).

Trees are ready to plant in spring 2026 after the last threat of freeze (in March). The planting of these trees is the final significant activity of this project. Trees have been spaded and after the threat of freeze, more trees will be spaded. We will propose to purchase a larger spade to collect the largest, healthiest specimens for consolidation because the USDA spade is not useful for moving the largest HLB tolerant specimens.

2. Please state what work is anticipated for next quarter:

Tree spading and removal of additional material for space for more Stage II trials. Planting of propagated trees in a consolidated block at the CREC.

3. Please state budget status (underspend or overspend, and why):

The budget is just a little overspent due to the no-cost extension, but is largely on track (86% time spent versus 94% funding expended). We are set to have expended all or nearly all funds in an efficient manner.



Development of Next-Generation SuperSour rootstocks with tolerance to HLB

Report Date: 01/08/2026   Project: 21-008   Year: 2025

Development of Next-Generation SuperSour rootstocks with tolerance to HLB

Report Date: 01/08/2026
Project: 21-008   Year: 2025
Category: Plant Improvement
Author: Kim Bowman
Sponsor: Citrus Research and Development Foundation



Development of Next-Generation SuperSour rootstocks with tolerance to HLB

Report Date: 01/08/2026   Project: 21-008   Year: 2025

Development of Next-Generation SuperSour rootstocks with tolerance to HLB

Report Date: 01/08/2026
Project: 21-008   Year: 2025
Category: Plant Improvement
Author: Kim Bowman
Sponsor: Citrus Research and Development Foundation



Rapid production of non-transgenic genome edited citrus plants

Report Date: 01/05/2026   Project: 24-015   Year: 2025

Rapid production of non-transgenic genome edited citrus plants

Report Date: 01/05/2026
Project: 24-015   Year: 2025
Category: Plant Improvement
Author: Manjul Dutt
Sponsor: Citrus Research and Development Foundation



Rapid production of non-transgenic genome edited citrus plants

Report Date: 01/05/2026   Project: 24-015   Year: 2025

Rapid production of non-transgenic genome edited citrus plants

Report Date: 01/05/2026
Project: 24-015   Year: 2025
Category: Plant Improvement
Author: Manjul Dutt
Sponsor: Citrus Research and Development Foundation



Evaluation of Potential HLB Tolerant Grapefruit Rootstock/Scion Combinations in Florida

Report Date: 12/16/2025   Project: 23-003   Year: 2025

Evaluation of Potential HLB Tolerant Grapefruit Rootstock/Scion Combinations in Florida

Report Date: 12/16/2025
Project: 23-003   Year: 2025
Percentage Completion: .99
Category: Plant Improvement
Author: Mark Ritenour
Sponsor: Citrus Research and Development Foundation



Evaluation of Potential HLB Tolerant Grapefruit Rootstock/Scion Combinations in Florida

Report Date: 12/16/2025   Project: 23-003   Year: 2025

Evaluation of Potential HLB Tolerant Grapefruit Rootstock/Scion Combinations in Florida

Report Date: 12/16/2025
Project: 23-003   Year: 2025
Category: Plant Improvement
Author: Mark Ritenour
Sponsor: Citrus Research and Development Foundation