Other


Evaluating the role of greasy spot and peel disorders in the greasy green defect on citrus fruit

Report Date: 05/05/2025   Project: 21-012   Year: 2025

Evaluating the role of greasy spot and peel disorders in the greasy green defect on citrus fruit

Report Date: 05/05/2025
Project: 21-012   Year: 2025
Percentage Completion: .90
Category: Other
Author: Megan Dewdney
Sponsor: Citrus Research and Development Foundation



Using Improved Diagnostics to Determine Citrus Blight Prevalence

Report Date: 04/25/2025   Project: 23-021   Year: 2025

Using Improved Diagnostics to Determine Citrus Blight Prevalence

Report Date: 04/25/2025
Project: 23-021   Year: 2025
Percentage Completion: 60%
Category: Other
Author: Ron Brlansy
Sponsor: Citrus Research and Development Foundation



CTV-T36 vector as a tool to induce efficient flowering in citrus seedlings

Report Date: 04/24/2025   Project: 21-014   Year: 2025

CTV-T36 vector as a tool to induce efficient flowering in citrus seedlings

Report Date: 04/24/2025
Project: 21-014   Year: 2025
Percentage Completion: .75
Category: Other
Author: Choaa El Mohtar
Sponsor: Citrus Research and Development Foundation



Title: Getting to the root of the problem: Managing Diaprepes root weevil on trees with HLB

Report Date: 04/03/2025   Project: 22-013   Year: 2025

Title: Getting to the root of the problem: Managing Diaprepes root weevil on trees with HLB

Report Date: 04/03/2025
Project: 22-013   Year: 2025
Percentage Completion: 0.8
Category: Other
Author: Lauren Diepenbrock
Sponsor: Citrus Research and Development Foundation



Exploring the HLB control potential of a new citrus-infecting virus, NMV-M/CFL, and its expression system

Report Date: 03/31/2025   Project: 23-028   Year: 2025

Exploring the HLB control potential of a new citrus-infecting virus, NMV-M/CFL, and its expression system

Report Date: 03/31/2025
Project: 23-028   Year: 2025
Percentage Completion: .82
Category: Other
Author: YongPing Duan
Sponsor: Citrus Research and Development Foundation

1. Please state project objectives and what work was done this quarter to address them:

The objectives of this research are
1) to reveal the distribution and stability of NMV-M/CFL in in citrus plants: We have inoculated sweet orange, lemon, grapefruit, pumelo, and Kumquat with NMV-M/CFL, and using graft-based propagated NMV-M-infected plants. The virus is systemic infected all citrus plants with different titers of the virus. However, like HLB bacterium, the virus tended to be unevenly distributed in citrus plants. We also surveyed our existing plot of research field with NMV-M, and revealed the virus was not wide-spread. We have identified better combination of citrus genotypes such as new bud-sport selections of grapefruit and Valencia, which yielded better protection with NMV-M infection.

2) to propagate the NMV-M/CFL-infected scions and rootstocks and evaluate their HLB-prevention and curative potentials in greenhouses and fields: NMV-M infected citrus germplasm was propagated to different grapefruit, Valencia, Cara Cara, and evaluated in USHRL greenhouse and field. Currently, over 30 of the new planting with NMV-M infected plants are in the USHRL research farm. We believe that some of our six-seven years old field trial of grapefruits is better than others may attribute to NMV-M/CFL infection. Our new planting with NMV-M continued to show better outcome than control. We tried to use MNV-M infected sticks to graft-inoculated to existing matured Valencia citrus plants with very low rate of success. We have applied for a permit from APHIS to allow us to use agrobacterium-mediated inoculation to inoculate NMV-M infectious clone into existing citrus trees.

3) We tried to use the Asian citrus psyllids to transmit the NMV-M/CFL under control conditions. Although we can detect the NMV-M/CFL in different generation of psyllids after the first generation had been reared on the NMV-M/CFL infected plants. Its transmission capability remains to be seen in a longer period.

2. Please state what work is anticipated for next quarter:
We will continue to evaluate the old and newly planted NMV-M/infected grapefruits and sweet orange, respectively in the fields, and the psyllid transmission study. we will temporarily conclude this one-year project. 3. Please state budget status (underspend or overspend, and why): Due to hiring freeze, we were not able to hire an employee, therefore the budget was underspend.

3. Please state budget status (underspend or overspend, and why):

Due to hiring freeze, we were not able to hire an employee, therefore the budget was under spended.



CTV-T36 vector as a tool to induce efficient flowering in citrus seedlings

Report Date: 01/27/2025   Project: 21-014   Year: 2024

CTV-T36 vector as a tool to induce efficient flowering in citrus seedlings

Report Date: 01/27/2025
Project: 21-014   Year: 2024
Percentage Completion: 0.65
Category: Other
Author: Choaa El Mohtar
Sponsor: Citrus Research and Development Foundation



CTV-T36 vector as a tool to induce efficient flowering in citrus seedlings

Report Date: 01/27/2025   Project: 21-014   Year: 2024

CTV-T36 vector as a tool to induce efficient flowering in citrus seedlings

Report Date: 01/27/2025
Project: 21-014   Year: 2024
Percentage Completion: .70
Category: Other
Author: Choaa El Mohtar
Sponsor: Citrus Research and Development Foundation



Using Improved Diagnostics to Determine Citrus Blight Prevalence

Report Date: 01/24/2025   Project: 23-021   Year: 2024

Using Improved Diagnostics to Determine Citrus Blight Prevalence

Report Date: 01/24/2025
Project: 23-021   Year: 2024
Percentage Completion: 0.5
Category: Other
Author: Ron Brlansy
Sponsor: Citrus Research and Development Foundation



Developing near and long-term management strategies for Lebbeck mealybug (Nipaecoccus viridis) in Florida Citrus

Report Date: 01/15/2025   Project: 20_002C   Year: 2024

Developing near and long-term management strategies for Lebbeck mealybug (Nipaecoccus viridis) in Florida Citrus

Report Date: 01/15/2025
Project: 20_002C   Year: 2024
Percentage Completion: 0.85
Category: Other
Author: Lauren Diepenbrock
Sponsor: Citrus Research and Development Foundation



Evaluating the role of greasy spot and peel disorders in the greasy green defect on citrus fruit

Report Date: 01/15/2025   Project: 21-012   Year: 2024

Evaluating the role of greasy spot and peel disorders in the greasy green defect on citrus fruit

Report Date: 01/15/2025
Project: 21-012   Year: 2024
Percentage Completion: 0.8
Category: Other
Author: Megan Dewdney
Sponsor: Citrus Research and Development Foundation



Getting to the root of the problem: Managing Diaprepes root weevil on trees with HLB

Report Date: 01/15/2025   Project: 20_013   Year: 2024

Getting to the root of the problem: Managing Diaprepes root weevil on trees with HLB

Report Date: 01/15/2025
Project: 20_013   Year: 2024
Percentage Completion: 0.75
Category: Other
Author: Lauren Diepenbrock
Sponsor: Citrus Research and Development Foundation



Developing near and long-term management strategies for Lebbeck mealybug (Nipaecoccus viridis) in Florida Citrus

Report Date: 01/15/2025   Project: 20_002C   Year: 2024

Developing near and long-term management strategies for Lebbeck mealybug (Nipaecoccus viridis) in Florida Citrus

Report Date: 01/15/2025
Project: 20_002C   Year: 2024
Percentage Completion: 0.87
Category: Other
Author: Lauren Diepenbrock
Sponsor: Citrus Research and Development Foundation



Developing management for Bulimulus bonariensis in Florida citrus

Report Date: 01/13/2025   Project: 22_014   Year: 2024

Developing management for Bulimulus bonariensis in Florida citrus

Report Date: 01/13/2025
Project: 22_014   Year: 2024
Percentage Completion: 0.7
Category: Other
Author: Lauren Diepenbrock
Sponsor: Citrus Research and Development Foundation



Evaluating the role of greasy spot and peel disorders in the greasy green defect on citrus fruit

Report Date: 11/26/2024   Project: 21-012   Year: 2024

Evaluating the role of greasy spot and peel disorders in the greasy green defect on citrus fruit

Report Date: 11/26/2024
Project: 21-012   Year: 2024
Percentage Completion: 0.74
Category: Other
Author: Megan Dewdney
Sponsor: Citrus Research and Development Foundation



A combined approach to reduce CLas and reverse symptoms in orchard trees

Report Date: 11/01/2024   Project: #23-044   Year: 2024

A combined approach to reduce CLas and reverse symptoms in orchard trees

Report Date: 11/01/2024
Project: #23-044   Year: 2024
Percentage Completion: 0.5
Category: Other
Author: Feng Gao
Sponsor: Citrus Research and Development Foundation